Actinomycin D

Research use only, not for human use.

A chemotherapy agent that has the ability to inhibit transcription.

A chemotherapy agent that has the ability to inhibit transcription; binds to DNA at the transcription initiation complex and preventing elongation of RNA chain by RNA polymerase; can also interfere with DNA replication; leads to the accumulation of transcriptionally active p53 in the nucleus of HeLa, CaSki, and SiHa cells.(In Vitro):Actinomycin D (Dactinomycin) is an inhibitor of DNA transcription and replication. Actinomycin D markedly reduces the vascular smooth muscle cells (SMC) proliferation via the inhibition of BrdU incorporation at 80 nM. This is further supported by the G1-phase arrest using a flowcytometric analysis. Actinomycin D is extremely potent with an inhibitory concentration IC50 at 0.4 nM, whereas the lethal dose LD50 is at 260 microM. The protein expression levels of proliferating cell nuclear antigen (PCNA), focal adhesion kinase (FAK), and Raf are all suppressed by Actinomycin D. Extracellular signal-regulated kinases (Erk) involved in cell-cycle arrest are found to increase by Actinomycin D.(In Vivo):The pluronic gel containing 80 nM and 80 μM Actinomycin D (Dactinomycin) is applied topically to surround the rat carotid adventitia, the thickness of neointima is substantially reduced (45 and 55%, respectively). Mice in the Actinomycin D and NSC 118218 group lives significantly longer than the control group with P values of <0.001 and 0.007, respectively. Interestingly, single treatment with Actinomycin D is superior to NSC 118218 regarding overall survival (P=0.026).

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Dactinomycin | Actinomycin IV

Cell Cycle/DNA Damage

DNA/RNA Synthesis

DNA/RNA Synthesis

Cancer

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50-76-0

1255.417

C62H86N12O16

>98% (HPLC)

DMSO: ≥ 27 mg/mL; DMSO: 250 mg/mL (Need ultrasonic); Methanol: 6.2 mg/mL; Methanol: 20 mg/mL (Need ultrasonic); H2O: < 12.5 mg/mL

O=C(N[C@@H]1C(N[C@@H](C(N2[C@]([H])(C(N(CC(N([C@H](C(O[C@@H]1C)=O)C(C)C)C)=O)C)=O)CCC2)=O)C(C)C)=O)C3=C(C(C(C)=C4OC5=C(C(C(N[C@@H]([C@@H](C)O6)C(N[C@H](C(C)C)C(N7CCC[C@@]7([H])C(N(C)CC(N(C)[C@@H](C(C)C)C6=O)=O)=O)=O)=O)=O)=CC=C5C)N=C43)=O)N

Actinomycin D

(-20℃)

With Ice Pack

≥ 2 years